e el r0868  (Elabscience Biotechnology)

Journal: Molecular Medicine Reports

Article Title: Ferroptosis contributes to hemolytic hyperbilirubinemia‑induced brain damage in vivo and in vitro

doi: 10.3892/mmr.2023.13123

Figure Lengend Snippet: Hemolytic hyperbilirubinemia-induced brain damage in neonatal Sprague-Dawley rats. Serum levels of (A) S100B and (B) neuron-specific enolase. (C) Tissue iron levels in the brain. Redox imbalances assessed by measuring levels of (D) GSH, (E) GSSG, (F) GSH/GSSG, (G) SOD and (H) MDA. n=6. *P<0.05; **P<0.01; ***P<0.001. S100B, S100 calcium-binding protein B; GSH, glutathione; GSSG, glutathione disulfide; SOD, superoxide dismutase; MDA, malondialdehyde; PHZ, phenylhydrazine; ns, not significant.

Article Snippet: Serum levels of S100B were assessed using the Rat S100B ELISA kit (cat. no. E-EL-R0868; Elabscience Biotechnology, Inc.), and those of serum NSE were assessed using the Rat NSE ELISA Kit (cat. no. E-EL-R0058c; Elabscience Biotechnology, Inc.) according to the manufacturer's instructions.

Techniques: Binding Assay

Journal: Molecular Medicine Reports

Article Title: Ferroptosis contributes to hemolytic hyperbilirubinemia‑induced brain damage in vivo and in vitro

doi: 10.3892/mmr.2023.13123

Figure Lengend Snippet: DFO pretreatment alleviates hemolytic hyperbilirubinemia-induced brain damage in neonatal Sprague-Dawley rats. Serum levels of (A) S100B and (B) neuron-specific enolase. (C) Levels of iron in the brain. Redox imbalances assessed by measuring the levels of (D) GSH, (E) GSSG, (F) GSH/GSSG, (G) SOD and (H) MDA. n=6. *P<0.05; **P<0.01; ***P<0.001. DFO, deferoxamine; S100B, S100 calcium-binding protein B; GSH, glutathione; GSSG, glutathione disulfide; SOD, superoxide dismutase; MDA, malondialdehyde; PHZ, phenylhydrazine; ns, not significant.

Article Snippet: Serum levels of S100B were assessed using the Rat S100B ELISA kit (cat. no. E-EL-R0868; Elabscience Biotechnology, Inc.), and those of serum NSE were assessed using the Rat NSE ELISA Kit (cat. no. E-EL-R0058c; Elabscience Biotechnology, Inc.) according to the manufacturer's instructions.

Techniques: Binding Assay

Journal: iScience

Article Title: Optimal inhaled oxygen and carbon dioxide concentrations for post-cardiac arrest cerebral reoxygenation and neurological recovery

doi: 10.1016/j.isci.2023.108476

Figure Lengend Snippet: Baseline characteristics, peri-resuscitation variables, and outcomes of randomized animals

Article Snippet: ELISA kits of NSE (#E-EL-R0058; Elabscience Biotechnology, Houston, TX), S100β (#E-EL-R0868; Elabscience Biotechnology), and cardiac troponin I (#ab246529; Abcam, Cambridge, UK) were used to determine their plasma levels in association with brain and heart injuries based on manufacturers instructions.

Techniques: Enzyme-linked Immunosorbent Assay, TUNEL Assay, Staining

Journal: iScience

Article Title: Optimal inhaled oxygen and carbon dioxide concentrations for post-cardiac arrest cerebral reoxygenation and neurological recovery

doi: 10.1016/j.isci.2023.108476

Figure Lengend Snippet: ELISA and TBARS assay at 24 h post-ROSC (A) NSE levels. (B) S100β levels. (C) MDA: levels. (D) Cardiac troponin I levels. Black: 50% iO 2 group; pink: 88% iO 2 group; green: 50% iO 2 + 12% iCO 2 group; purple: 88% iO 2 + 12% iCO 2 group. The neuronal injuries were evaluated using the NSE and S100β plasma concentrations. Oxidative injury was assessed using the MDA level in the hippocampus. Data are presented as a boxplot. Pairwise comparisons between experimental groups were performed using post hoc Dunn’s test. ELISA, enzyme-linked immunosorbent assay; iCO 2 , inhaled carbon dioxide; iO 2 , inhaled oxygen; KW: Kruskal-Wallis; MDA, malondialdehyde; NSE, neuron-specific enolase; ROSC, return of spontaneous circulation; TBARS, thiobarbituric acid reactive substance.

Article Snippet: ELISA kits of NSE (#E-EL-R0058; Elabscience Biotechnology, Houston, TX), S100β (#E-EL-R0868; Elabscience Biotechnology), and cardiac troponin I (#ab246529; Abcam, Cambridge, UK) were used to determine their plasma levels in association with brain and heart injuries based on manufacturers instructions.

Techniques: Enzyme-linked Immunosorbent Assay, TBARS Assay, Clinical Proteomics

Journal: iScience

Article Title: Optimal inhaled oxygen and carbon dioxide concentrations for post-cardiac arrest cerebral reoxygenation and neurological recovery

doi: 10.1016/j.isci.2023.108476

Figure Lengend Snippet:

Article Snippet: ELISA kits of NSE (#E-EL-R0058; Elabscience Biotechnology, Houston, TX), S100β (#E-EL-R0868; Elabscience Biotechnology), and cardiac troponin I (#ab246529; Abcam, Cambridge, UK) were used to determine their plasma levels in association with brain and heart injuries based on manufacturers instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, TBARS Assay, TUNEL Assay, Software